Agglutination-PCR

From Wikipedia the free encyclopedia

Agglutination-PCR (ADAP) is an ultrasensitive solution-phase method for detecting antibodies.[1] Antibodies bind to and agglutinate synthetic antigen–DNA conjugates, enabling ligation of the DNA strands and subsequent quantification by qPCR. Like other Immuno-PCR (IPCR) detection methods[2][3] ADAP combines the specificity of antibody-antigen recognition and the sensitivity of PCR. ADAP detects zepto- to attomoles of antibodies in 2 μL of sample with a dynamic range spanning 5–6 orders of magnitude. For example, ADAP allows to detect anti-thyroglobulin autoantibodies from human patient plasma with a 1000-fold increased sensitivity over an FDA-approved radioimmunoassay. ADAP also allows to simultaneously detect multiple antibodies in one experiment, much more than ELISA or radioimmunoassay.[citation needed]

The study published in the ACS Central Science journal mentioned that this testing method is 10,000 times more effective than the existing diagnostic techniques.[4][5]

Another advantage of ADAP method is the simplicity. It can be adapted to such a cheap equipment as, for example, SlipChip.[6][7] It does not require complex and expensive equipment, it does not require working with hazardous radioactive reagents.

References[edit]

  1. ^ Tsai, Cheng-Ting; Robinson, Peter V.; Spencer, Carole A.; Bertozzi, Carolyn R. (2016). "Ultrasensitive Antibody Detection by Agglutination-PCR (ADAP)". ACS Central Science. 2 (3): 139–147. doi:10.1021/acscentsci.5b00340. PMC 4819452. PMID 27064772.
  2. ^ Chang, Le; Li, Jinming; Wang, Lunan (2016). "Immuno-PCR: An ultrasensitive immunoassay for biomolecular detection". Analytica Chimica Acta. 910: 12–24. Bibcode:2016AcAC..910...12C. doi:10.1016/j.aca.2015.12.039. PMID 26873464.
  3. ^ Spengler, Mark; Adler, Michael; Niemeyer, Christof M. (2015). "Highly sensitive ligand-binding assays in pre-clinical and clinical applications: Immuno-PCR and other emerging techniques". The Analyst. 140 (18): 6175–6195. Bibcode:2015Ana...140.6175S. doi:10.1039/C5AN00822K. PMID 26196036.
  4. ^ University, Stanford (2016-03-10). "Stanford chemists develop an ultra-sensitive test for cancers, HIV". Stanford News. Retrieved 2017-09-12.
  5. ^ "New Ultra-Sensitive Test Up to 10,000 Times More Effective In Detecting HIV, Cancer". Tech Times. 2016-03-21. Retrieved 2017-09-12.
  6. ^ Shen, Feng; Du, Wenbin; Kreutz, Jason E.; Fok, Alice; Ismagilov, Rustem F. (2010). "Digital PCR on a Slip Chip". Lab on a Chip. 10 (20): 2666–2672. doi:10.1039/c004521g. PMC 2948063. PMID 20596567.
  7. ^ Rodriguez-Manzano, Jesus; Karymov, Mikhail A.; Begolo, Stefano; Selck, David A.; Zhukov, Dmitriy V.; Jue, Erik; Ismagilov, Rustem F. (2016). "Reading Out Single-Molecule Digital RNA and DNA Isothermal Amplification in Nanoliter Volumes with Unmodified Camera Phones". ACS Nano. 10 (3): 3102–3113. doi:10.1021/acsnano.5b07338. PMC 4819493. PMID 26900709.

Further reading[edit]

  • Alexander, L., Rasmus, B., Charlotte, B., & Daniel, A. (2023). Antibody detection by agglutination-PCR (ADAP) assays for the analysis of tissue transglutaminase autoantibodies in celiac disease. Journal of Immunological Methods, 113502. PMID 37257686 doi:10.1016/j.jim.2023.113502
  • Lind, A., de Jesus Cortez, F., Ramelius, A., Bennet, R., Robinson, P. V., Seftel, D., ... & Lernmark, Å. (2022). Multiplex agglutination-PCR (ADAP) autoantibody assays compared to radiobinding autoantibodies in type 1 diabetes and celiac disease. Journal of Immunological Methods, 506, 113265. PMID 35358496 doi:10.1016/j.jim.2022.113265
  • de Jesus Cortez, F., Gebhart, D., Tandel, D., Robinson, P. V., Seftel, D., Wilson, D. M., ... & Tsai, C. T. (2022). Automation of a multiplex agglutination-PCR (ADAP) type 1 diabetes (T1D) assay for the rapid analysis of islet autoantibodies. SLAS technology, 27(1), 26-31. PMID 35058202 doi:10.1016/j.slast.2021.10.001
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