Vent DNA polymerase

Vent polymerase is a thermostable archean DNA polymerase used for the polymerase chain reaction. It was isolated from the thermophile Thermococcus litoralis.^[1]

References[edit]

^ Vigneault F, Drouin R (2005). "Optimal conditions and specific characteristics of Vent exo- DNA polymerase in ligation-mediated polymerase chain reaction protocols". Biochem Cell Biol. 83 (2): 147–65. doi:10.1139/o04-134. PMID 15864324.

v t e Polymerase chain reaction techniques
Procedure	Initialization Cycle Denaturation Annealing Elongation Final elongation Final hold
Polymerase	Klenow T4 and T7 Taq Tth Pfu Vent Pwo Tli Tfu
Optimization and variants	Quantitative polymerase chain reaction (qPCR) Reverse transcription polymerase chain reaction (RT-PCR) Inverse polymerase chain reaction (inverse PCR) Nested polymerase chain reaction (nested PCR) Touchdown polymerase chain reaction Hot start PCR Overlap extension polymerase chain reaction (OE-PCR) Multiplex polymerase chain reaction (multiplex PCR) Multiplex ligation-dependent probe amplification (MLPA) Co-amplification at lower denaturation temperature PCR (COLD-PCR) Random amplification of polymorphic DNA (RAPD)
History and people	Kary Mullis Kjell Kleppe Alice Chien