4-hydroxyphenylacetate 1-monooxygenase

In enzymology, a 4-hydroxyphenylacetate 1-monooxygenase (EC 1.14.13.18) is an enzyme that catalyzes the chemical reaction

4-hydroxyphenylacetate + NAD(P)H + H⁺ + O₂ ${\displaystyle \rightleftharpoons }$ homogentisate + NAD(P)+ + H₂O

The 5 substrates of this enzyme are 4-hydroxyphenylacetate, NADH, NADPH, H⁺, and O₂, whereas its 4 products are homogentisate, NAD⁺, NADP⁺, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is 4-hydroxyphenylacetate,NAD(P)H:oxygen oxidoreductase (1-hydroxylating). Other names in common use include 4-hydroxyphenylacetate 1-hydroxylase, 4-hydroxyphenylacetic 1-hydroxylase, and 4-HPA 1-hydroxylase. This enzyme participates in tyrosine metabolism.. It employs one cofactor, FAD.

• Hareland WA, Crawford RL, Chapman PJ, Dagley S (1975). "Metabolic function and properties of 4-hydroxyphenylacetic acid 1-hydroxylase from Pseudomonas acidovorans". J. Bacteriol. 121 (1): 272–85. PMC 285641. PMID 234937.