Stanley Fields (biologist)

Stan Fields
Born
Stanley Fields
Alma materUniversity of Cambridge
Known forTwo-hybrid screening
Scientific career
Institutions
ThesisSequence analysis of influenza virus RNA (1981)
Website

Stanley Fields is an American biologist best known for developing the yeast two hybrid method for identifying protein–protein interactions.[1] He is currently a professor of Genome Sciences at the University of Washington and Howard Hughes Medical Institute Investigator,[2][3] and previously served as chair of the Department of Genome Sciences.[4]

Education

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Fields was educated at the University of Cambridge where he was awarded a Doctor of Philosophy in 1981 for research carried out in the Laboratory of Molecular Biology with Greg Winter and George Brownlee.[5][6]

Research

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Fields developed the yeast two-hybrid system in 1989,[1] which has been widely used by Fields[7][8][9][10] and others to identify protein-protein interactions in various organisms and biological contexts.

Along with Matt Kaeberlein and Brian Kennedy, in later work Fields has carried out genome-wide screens for aging genes in yeast. Kaeberlein and co-workers have questioned the hypothesis that lifespan extension from caloric restriction is mediated by Sirtuins.[11] Instead Kaeberlein, Fields, and Kennedy have proposed that caloric restriction increases lifespan by decreasing the activity of the Target of Rapamycin (TOR) kinase.[12]

Honors and awards

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References

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  1. ^ a b Fields, S.; Song, O. (1989). "A novel genetic system to detect protein-protein interactions". Nature. 340 (6230): 245–246. Bibcode:1989Natur.340..245F. doi:10.1038/340245a0. PMID 2547163. S2CID 4320733.
  2. ^ Fields, Stanley (2014). "Would Fred Sanger Get Funded Today?". Genetics. 197 (2): 435–439. doi:10.1534/genetics.114.165134. PMC 4063904. PMID 24939989.
  3. ^ Stanley Fields's publications indexed by the Scopus bibliographic database. (subscription required)
  4. ^ "UW Genome Sciences: Stanley Fields". www.gs.washington.edu. Retrieved 2023-08-14.
  5. ^ Winter, G.; Fields, S.; Brownlee, G. G. (1981). "Nucleotide sequence of the haemagglutinin gene of a human influenza virus H1 subtype". Nature. 292 (5818): 72–75. Bibcode:1981Natur.292...72W. doi:10.1038/292072a0. PMID 7278968. S2CID 4312205.
  6. ^ Fields, S.; Winter, G.; Brownlee, G. G. (1981). "Structure of the neuraminidase gene in human influenza virus A/PR/8/34". Nature. 290 (5803): 213–217. Bibcode:1981Natur.290..213F. doi:10.1038/290213a0. PMID 7010182. S2CID 8051512.
  7. ^ Bartel, P. L.; Roecklein, J. A.; SenGupta, D.; Fields, S. (January 1996). "A protein linkage map of Escherichia coli bacteriophage T7". Nature Genetics. 12 (1): 72–77. doi:10.1038/ng0196-72. ISSN 1061-4036. PMID 8528255.
  8. ^ Iwabuchi, K.; Li, B.; Bartel, P.; Fields, S. (June 1993). "Use of the two-hybrid system to identify the domain of p53 involved in oligomerization". Oncogene. 8 (6): 1693–1696. ISSN 0950-9232. PMID 8502489.
  9. ^ Schwikowski, B.; Uetz, P.; Fields, S. (December 2000). "A network of protein-protein interactions in yeast". Nature Biotechnology. 18 (12): 1257–1261. doi:10.1038/82360. ISSN 1087-0156. PMID 11101803. S2CID 3009359.
  10. ^ Iwabuchi, K.; Bartel, P. L.; Li, B.; Marraccino, R.; Fields, S. (1994-06-21). "Two cellular proteins that bind to wild-type but not mutant p53". Proceedings of the National Academy of Sciences of the United States of America. 91 (13): 6098–6102. Bibcode:1994PNAS...91.6098I. doi:10.1073/pnas.91.13.6098. ISSN 0027-8424. PMC 44145. PMID 8016121.
  11. ^ Kaeberlein, M.; Kirkland, K. T.; Fields, S.; Kennedy, B. K. (2004). "Sir2-Independent Life Span Extension by Calorie Restriction in Yeast". PLOS Biology. 2 (9): e296. doi:10.1371/journal.pbio.0020296. PMC 514491. PMID 15328540.
  12. ^ Kaeberlein, M; Powers Rw, III; Steffen, K. K.; Westman, E. A.; Hu, D; Dang, N; Kerr, E. O.; Kirkland, K. T.; Fields, S; Kennedy, B. K. (2005). "Regulation of yeast replicative life span by TOR and Sch9 in response to nutrients". Science. 310 (5751): 1193–6. Bibcode:2005Sci...310.1193K. doi:10.1126/science.1115535. PMID 16293764. S2CID 42188272.
  13. ^ "Past Winners".


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