| Route | Local Name(s) | Type | Northern/Western Terminus | Southern/Eastern Terminus | Communities | Comments |
| | Snyder's Road, Waterloo Street, Huron Street, Wilmot-Easthope Road | | 110m South of Huron Road | RR 6 (Gingerich Road) | New Hamburg, Baden | |
| | Walker Road | | Highway 7 & 8 | Oxford-Waterloo Road | | Minor rural route |
| | Huron Street, Peel Street, Bleams Road, Ottawa Street | Arterial road | RR 1 (Waterloo Street) | RR 54 (Lackner Boulevard), Keewatin Avenue | New Hamburg, Mannheim, Kitchener | Intersects the Conestoga Parkway twice. Ottawa Street proposed extension from Keewatin Avenue to RR 17 (Fountain Street Breslau) |
| | Manser Road, William Hastings Line, Hutchison Road, Queen's Bush Road, Nafziger Road | | RR 86 | Highway 7 & 8 | Wellesley, Linwood | |
| | Gingerich Road, Snyders Road, Highland Road, Queen Street, Benton Street, Frederick Street | Arterial road | RR 51 (Foundry Street) | RR 61 (Bruce Street) | Baden, Kitchener | |
| | Hutchison Road, Perth Line | | RR 5 (Queen's Bush Road) | Road 105 | Wellesley | |
| | King Street, Weber Street, Shantz Hill Road, Fountain Street, Coronation Boulevard, Dundas Street | | RR 17 (Sawmill Road) | RR 43 (Branchton Road) / Highway 8 | St. Jacobs, Waterloo, Kitchener, Cambridge | Formerly Highway 8 between Freeport Diversion and RR 43 (Branchton Road) |
| | Wilmot-Easthope Road, Erb's Road, Erb Street, Caroline Street, Bridgeport Road | | Perth County Road 43 | RR 29 (Lancaster Street) | St. Agatha, Waterloo, Bridgeport | |
| Waterloo Regional Road 10 |
| Herrgott Road | Arterial road | RR 86 (Line 86) | RR 15 (Lobsinger Line) | St. Clements, Wallenstein | |
| Waterloo Regional Road 11 |
| William Hastings Line | Rural route | Road 116 | RR 5 (Manser Road) | Perth County boundary | |
| Waterloo Regional Road 12 |
| Gerber Road, Notre Dame Drive, Queen Street, Bridge Street, New Dundee Road | | RR 5 (Nafziger Road) | RR 28 (Homer Watson Boulevard) | Wellesley, St. Agatha, New Dundee, Doon | |
| Waterloo Regional Road 14 |
| Kressler Road, Weimar Line, Moser-Young Road | | RR 12 (Gerber Road/Notre Dame Drive) | RR 70 (Erbsville Road) | Bamberg | |
| Waterloo Regional Road 15 |
| Lobsinger Line, King Street | Arterial road | RR 5 (Hutchison Road) | Dixon Street/Montgomery Road | St. Clements, Heidelberg, Waterloo, Kitchener | |
| Waterloo Regional Road 16 |
| Kressler Road | | RR 17 (Hawkesville Road) | RR 70 (Erbsville Road) | Heidelberg, Erbsville | |
| Waterloo Regional Road 17 |
| Fountain Street, Ebycrest Road, Sawmill Road, Northside Drive, Hawkesville Road, Ament Line | | RR 5 (Manser Road) | Highway 8 | St. Jacobs, Conestogo, Bloomingdale, Breslau, Cambridge | |
| Waterloo Regional Road 19 |
| Floradale Road | | Florapine Road | RR 86 | Floradale | |
| Waterloo Regional Road 20 |
| Bloomingdale Road | | RR 52 (Bridge Street) | RR 17 (Ebycrest Road/Sawmill Road) | Bridgeport | |
| Waterloo Regional Road 21 |
| Arthur Street | | Floradale Road | RR 85 (Listowel Road) | Elmira, North Woolwich | |
| Waterloo Regional Road 22 |
| Northfield Drive | | Wellington County limits | RR 15 (King Street) | Waterloo, Conestogo | |
| Waterloo Regional Road 23 |
| Katherine Street | | Woolwich-Pilkington Townline | RR 17 (Sawmill Road) | Winterbourne, Zuber Corners | |
| Waterloo Regional Road 24 |
| Hespeler Road, Water Street, Ainslie Street | | Wellington County limits | RR 81 (McQueen Shaver Boulevard) / Highway 24 | Glenchristie, Cambridge | Formerly Highway 24 |
| Waterloo Regional Road 25 |
| Maryhill Road | | Woolwich-Guelph Townline | RR 26 (St. Charles Street) | Maryhill, Bloomingdale | |
| Waterloo Regional Road 26 |
| St. Charles Street | | RR 17 (Sawmill Road) | RR 25 (Maryhill Road) | Maryhill, Bloomingdale | |
| Waterloo Regional Road 27 |
| Clyde Road, Samuelson Street, Beverly Street, Wellington Street, Main Street, Queen's Square, North Square, South Square | | RR 42 (George Street) | Village Road | Cambridge, Clyde | |
| Waterloo Regional Road 28 |
| Homer Watson Boulevard, Fountain Street | | RR 4 (Ottawa Street) | RR 8 (Shantz Hill Road) | Cambridge, Doon, Kitchener | Includes small section of Huron Road from on and off ramps to Homer Watson Boulevard. |
| Waterloo Regional Road 29 |
| Lancaster Street | | RR 52 (Bridge Street) | RR 55 (Victoria Street) | Kitchener, Bridgeport | |
| Waterloo Regional Road 30 |
| Shantz Station Road | | RR 26 (St. Charles Street) | RR 31 (Kossuth Road) | Kossuth | |
| Waterloo Regional Road 31 |
| Kossuth Road | | RR 17 (Fountain Street) | RR 24 (Hespeler Road) | Kossuth | |
| Waterloo Regional Road 33 |
| Townline Road | | Wellington County Road 34 | Gore Road | Cambridge | |
| Waterloo Regional Road 36 |
| Franklin Boulevard | | Highway 401 | RR 81 (McQueen Shaver Boulevard) | Cambridge | |
| Waterloo Regional Road 37 |
| Allen Street | | RR 9 (Caroline Street) | RR 15 (King Street) | Uptown Waterloo | |
| Waterloo Regional Road 38 |
| Sportsworld Drive, Maple Grove Road | | RR 8 (King Street) | RR 24 (Hespeler Road) | Kitchener | |
| Waterloo Regional Road 39 |
| Eagle Street, Pinebush Road | Arterial road | RR 8 (King Street) | RR 33 (Townline Road) | Cambridge | |
| Waterloo Regional Road 41 |
| Bishop Street | | RR 8 (King Street) | RR 36 (Franklin Boulevard) | Cambridge | |
| Waterloo Regional Road 42 |
| Blair Road, George Street | | RR 28 (Fountain Street) | RR 75 (St. Andrews Street) | Cambridge | |
| Waterloo Regional Road 43 |
| Myers Road, Branchton Road | | Highway 24 | Brant County Road 144 | Cambridge, Littles Corners, Branchton | |
| Waterloo Regional Road 46 |
| Roseville Road | | Regional Road 58 | Cambridge city limits | Roseville, Orrs Lake | Shares roadway with Regional Road 71 between Dickie Settlement Road and Edworthy Sideroad |
| Waterloo Regional Road 47 |
| Dumfries Road | | Regional Road 46 | Regional Road 49 | Wrigley | |
| Waterloo Regional Road 49 |
| Wrigley Road, Scott Street, Main Street, Stanley Street | | Regional Road 58 | Regional Road 75 | Wrigley, Ayr | |
| Waterloo Regional Road 50 |
| Northfield Drive, Westmount Road | | Regional Road 15 | Regional Road 58 | Kitchener, Waterloo | |
| Waterloo Regional Road 51 |
| Foundry Street, Wilmot Centre Road | | Regional Road 1 | Regional Road 4 | Baden, Wilmot Centre | |
| Waterloo Regional Road 52 |
| Bridge Street | | RR 22 (Northfield Drive) | Regional Road 17 | Waterloo, Bridgeport | |
| Waterloo Regional Road 53 |
| Courtland Avenue, Fairway Road | | Regional Road 6 | RR 17 (Fountain Street) | Kitchener | |
| Waterloo Regional Road 54 |
| Lackner Boulevard | | Highway 7 | RR 53 (Fairway Road) | Kitchener | |
| Waterloo Regional Road 55 |
| Victoria Street | | RR 70 (Ira Needles Boulevard) | Highway 85, RR 17 (Fountain Street) | Kitchener | Continues past Highway 85 as both Highway 7 and RR 55 |
| Waterloo Regional Road 56 |
| Bleams Road, River Road | | RR 70 (Trussler Road), RR 8 (King Street) | RR 69 (Manitou Drive), RR 53 (Fairway Road) | Kitchener | To be connected from RR 8 to RR 69 in the near future. |
| Waterloo Regional Road 57 |
| University Avenue | Arterial road | RR 70 (Ira Needles Boulevard) | RR 52 (Bridge Street) | Waterloo | Name derives from the universities of Waterloo and Wilfrid Laurier, which it serves. |
| Waterloo Regional Road 58 |
| Bearinger Road, Fischer-Hallman Road, Roseville Road, Northumberland Street, Stanley Street, Swan Street | | RR 50 (Westmount Road) | Brant-Waterloo Road | Waterloo, Kitchener, Roseville, Ayr | |
| Waterloo Regional Road 61 |
| Bruce Street | | RR 55 (Victoria Street) | RR 6 (Frederick Street) | Kitchener | |
| Waterloo Regional Road 62 |
| Edna Street | | RR 55 (Victoria Street) | RR 6 (Frederick Street) | Kitchener | |
| Waterloo Regional Road 63 |
| Duke Street | | RR 68 (Francis Street) | RR 6 (Frederick Street) | Kitchener | |
| Waterloo Regional Road 64 |
| Charles Street | | RR 55 (Victoria Street) | RR 4 (Ottawa Street) | Kitchener | |
| Waterloo Regional Road 66 |
| Borden Avenue | | Unnamed laneway connecting Borden Avenue and Grenville Avenue | RR 64 (Charles Street) | Kitchener | |
| Waterloo Regional Road 67 |
| Hayward Avenue | | 230m West of RR 53 (Courtland Avenue) | RR 53 (Courtland Avenue) | Kitchener | |
| Waterloo Regional Road 68 |
| Francis Street | | RR 63 (Duke Street) | RR 15 (King Street) | Kitchener | |
| Waterloo Regional Road 69 |
| Manitou Drive | | RR 53 (Fairway Road) | RR 28 (Homer Watson Boulevard) | Kitchener | |
| Waterloo Regional Road 70 |
| Erbsville Road, Ira Needles Boulevard, Trussler Road | | RR 14 & 16 (Kressler Road) | Brant Waterloo Road | Erbsville, Waterloo, Kitchener, Mannheim, Plumtree | |
| Waterloo Regional Road 71 |
| Dickie Settlement Road, Roseville Road, Edworthy Sideroad | Rural route | Regional Road 28 | Regional Road 97 | North Dumfries | |
| Waterloo Regional Road 75 |
| Spragues Road, St. Andrews Street | | Regional Road 76 (Grand Avenue) | Brant-Waterloo Road | Cambridge | Formerly Highway 24A |
| Waterloo Regional Road 76 |
| Grand Avenue | | RR 75 (St. Andrews Street) | RR 97 (Cedar Street) | Cambridge | |
| Waterloo Regional Road 77 |
| Park Hill Road | | RR 42 (George Street) | RR 24 (Ainslie Street) | Cambridge | |
| Waterloo Regional Road 80 |
| Can-Amera Parkway | | Highway 24 | RR 33 (Townline Road) | Cambridge | |
| Waterloo Regional Road 81 |
| McQueen Shaver Boulevard | | Highway 24 | RR 36 (Franklin Boulevard) | Cambridge, North Dumfries | South boundary road, to be extended to RR 33 (Townline Road) |
| Waterloo Regional Road 85 |
| Listowel Road, Arthur Street South | Rural route | RR 86 | RR 15 (King Street) / Highway 85 (Conestoga Parkway) | Elmira | Formerly Highway 85 |
| Waterloo Regional Road 86 |
| Church Street, Line 86 | | Red Woods Drive | Sideroad 16 | Macton, Wallenstein, Elmira, Zuber Corners, Weissenburg | Formerly Highway 86 |
 | Waterloo Regional Road 97 |
| Cedar Creek Road, Cedar Street, Concession Street, Main Street, Old Beverly Road | | RR 70 (Trussler Road) | Hamilton city (old Wentworth County) limits | Cambridge, North Dumfries | Formerly Highway 97 |
