| # | Rider | Time |
| 1 |  Tatiana Guderzo (Italy) | 3h 33:25 |
| 2 |  Marianne Vos (Netherlands) | + 19 |
| 3 | Noemi Cantele (Italy) | + 19 |
| 4 |  Kristin Armstrong (United States) | + 19 |
| 5 |  Diana Žiliūtė (Lithuania) | + 1:07 |
| 6 |  Judith Arndt (Germany) | + 1:07 |
| 7 |  Erinne Willock (Canada) | + 1:07 |
| 8 |  Nicole Brändli (Switzerland) | + 1:07 |
| 9 |  Grace Verbeke (Belgium) | + 1:07 |
| 10 |  Catherine Cheatley (New Zealand) | + 1:07 |
| 11 |  Emma Johansson (Sweden) | + 1:07 |
| 12 |  Ruth Corset (Australia) | + 1:07 |
| 13 | Edita Pučinskaitė (Lithuania) | + 1:07 |
| 14 |  Emma Pooley (Great Britain) | + 1:07 |
| 15 | Evelyn Stevens (United States) | + 1:07 |
| 16 |  Linda Melanie Villumsen (Denmark) | + 3:02 |
| 17 |  Paulina Brzeźna (Poland) | + 3:41 |
| 18 | Mara Abbott (United States) | + 4:51 |
| 19 | Claudia Häusler (Germany) | + 5:51 |
| 20 |  Ana Garcia Antequera (Spain) | + 5:54 |
| 21 | Andrea Bosman (Netherlands) | + 6:54 |
| 22 | Ludivine Henrion (Belgium) | + 6:54 |
| 23 | Chantal Blaak (Netherlands) | + 6:54 |
| 24 |  Yuliya Martisova (Russia) | + 6:54 |
| 25 |  Christel Ferrier-Bruneau (France) | + 6:54 |
| 26 | Edwige Pitel (France) | + 6:54 |
| 27 | Elizabeth Armitstead (Great Britain) | + 6:54 |
| 28 | Sharon Laws (Great Britain) | + 6:54 |
| 29 | Trixi Worrack (Germany) | + 6:54 |
| 30 | Fabiana Luperini (Italy) | + 6:54 |
| 31 | Karine Gautard (France) | + 9:52 |
| 32 | Rasa Leleivytė (Lithuania) | + 10:11 |
| 33 | Kaytee Boyd (New Zealand) | + 10:11 |
| 34 |  Carla Swart (South Africa) | + 10:11 |
| 35 | Sophie Creux (France) | + 10:11 |
| 36 | Monika Grzebinoga (Poland) | + 10:11 |
| 37 | Marissa van der Merwe (South Africa) | + 10:11 |
| 38 |  Verónica Leal Balderas (Mexico) | + 10:11 |
| 39 |  Christiane Soeder (Austria) | + 10:11 |
| 40 | Tara Whitten (Canada) | + 10:11 |
| 41 |  Olena Oliinyk (Ukraine) | + 10:11 |
| 42 | Regina Bruins (Netherlands) | + 10:13 |
| 43 | Irene van den Broek (Netherlands) | + 10:13 |
| 44 | Julie Krasniak (France) | + 10:13 |
| 45 | Loes Gunnewijk (Netherlands) | + 10:13 |
| 46 | Monia Baccaille (Italy) | + 10:18 |
| 47 | Luisa Tamanini (Italy) | + 10:18 |
| 48 | Jeannie Longo-Ciprelli (France) | + 12:09 |
| 49 | Oxana Kozonchuk (Russia) | + 12:46 |
| 50 | Eivgenia Vysotska (Ukraine) | + 12:46 |
| 51 | Laura Lorenza Morfin Macouzet (Mexico) | + 12:46 |
| 52 | Tiffany Cromwell (Australia) | + 12:46 |
| 53 | Alyona Andruk (Ukraine) | + 12:46 |
| 54 | Patricia Schwager (Switzerland) | + 12:46 |
| 55 | Lieselot Decroix (Belgium) | + 12:46 |
| 56 | Daniela Pintarelli (Austria) | + 13:02 |
| DNF | Modesta Vžesniauskaitė (Lithuania) | |
| DNF |  Siobhan Horgan (Ireland) | |
| DNF | Rachel Mercer (New Zealand) | |
| DNF | Giuseppina Grassi Herrera (Mexico) | |
| DNF | Anna Evseeva (Russia) | |
| DNF | Małgorzata Jasińska (Poland) | |
| DNF | Nina Ovcharenko (Ukraine) | |
| DNF | Liesbet De Vocht (Belgium) | |
| DNF | Kimberly Anderson (United States) | |
| DNF | Giorgia Bronzini (Italy) | |
| DNF | Tina Liebig (Germany) | |
| DNF | Tatiana Antoshina (Russia) | |
| DNF | Jessie Daams (Belgium) | |
| DNF | Eneritz Iturriagaechevarria Mazaga (Spain) | |
| DNF | Edita Ungurytė (Lithuania) | |
| DNF | Inga Čilvinaitė (Lithuania) | |
| DNF | Anna Sanchis Chafer (Spain) | |
| DNF | Martine Bras (Netherlands) | |
| DNF | Joëlle Numainville (Canada) | |
| DNF | Svetlana Bubnenkova (Russia) | |
| DNF |  Ariadna Tudel Cuberes (Andorra) | |
| DNF | Nicole Cooke (Great Britain) | |
| DNF | Katie Colclough (Great Britain) | |
| DNF |  Sigrid Corneo (Slovenia) | |
| DNF | Vicki Whitelaw (Australia) | |
| DNF | Charlotte Becker (Germany) | |
| DNF | Jennifer Hohl (Switzerland) | |
| DNF | Trine Schmidt (Denmark) | |
| DNF | Emilie Aubry (Switzerland) | |
| DNF | Sylwia Kapusta (Poland) | |
| DNF | Polona Batagelj (Slovenia) | |
| DNF |  Rosane Kirch (Brazil) | |
| DNF | Marie Lindberg (Sweden) | |
| DNF | Emilia Fahlin (Sweden) | |
| DNF | Jessica Schneeberger (Switzerland) | |
| DNF |  Chanpeng Nontasin (Thailand) | |
| DNF |  Ester Alves (Portugal) | |
| DNF |  Fröydis Waerstad (Norway) | |
| DNF | Ina Teutenberg (Germany) | |
| DNF | Lucy Martin (Great Britain) | |
| DNF | Belén López Morales (Spain) | |
| DNF | Elena Novikova (Russia) | |
| DNF | Olivia Dillon (Ireland) | |
| DNF | Andrea Wölfer (Switzerland) | |
| DNF |  Martina Růžičková (Czech Republic) | |
| DNF |  Maja Marukic (Croatia) | |
| DNF |  Semra Yetiş (Turkey) | |
| DNF |  Kathryn Bertine (Saint Kitts and Nevis) | |
| DNF | Marina Boduljak (Croatia) | |
| DNF | Monrudee Chapookam (Thailand) | |
| DNF | Wilaiwan Kunlapha (Thailand) | |
| DNF | Thatsani Wichana (Thailand) | |
| DNF | Amber Neben (United States) | |
| DNF |  Dahlina Rosyida (Indonesia) | |
| DNF | Meredith Miller (United States) | |
| DNF | Rochelle Gilmore (Australia) | |
| DNF | Emma Mackie (Australia) | |
| DNF | Heather Logan (Canada) | |
| DNF | Monica Ceccon (Saint Kitts and Nevis) | |
| DNF | Blaža Klemenčič (Slovenia) | |
| DNF | Sara Mustonen (Sweden) | |
| DNF | Oksana Kashchyshyna (Ukraine) | |
| DNF | Alison Testroete (Canada) | |
| DNF |  Veronica Marcela Gerez (Argentina) | |
| DNF | Julie Beveridge (Canada) | |
| DNF | Marta Vila Josana Andreu (Spain) | |
| DNF | Ane Santesteban Gonzalez (Spain) | |
| DNF | Uênia Fernandes de Souza (Brazil) | |
| DNF | Viena Balen (Croatia) | |
| DNF | Alexis Rhodes (Australia) | |
| DNF | Nikki Harris (Great Britain) | |
Mendrisio (SWI)
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