David W. Wood

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David W. Wood
EducationRensselaer Polytechnic Institute (M.S. & Ph.D.), California Institute of Technology (Caltech, B.S.)
Scientific career
InstitutionsOhio State University, Princeton University, Amgen, Bristol Myers Squibb, CP Kelco
ThesisGeneration and application of a self-cleaving protein linker for use in single-step affinity fusion based protein purification (2000)
Doctoral advisorsGeorges Belfort, Marlene Belfort
Websitehttps://cbe.osu.edu/wood-laboratory-applied-protein-engineering

David W. Wood (born in 1967) is an American chemical engineer who is Professor of Chemical and Biomolecular Engineering at Ohio State University. Wood is also associated with the Department of Chemistry and Biochemistry and Molecular Biophysics Training Program.

Wood is best known for his work on self-removing affinity tag methods, which he first published in Nature Biotechnology[1] while a Ph.D. student at Rensselaer Polytechnic Institute. This method was also patented as a part of a collaboration with co-inventors at the Wadsworth Center of the New York State Department of Health and Rensselaer Polytechnic Institute, including Marlene Belfort, Georges Belfort, Victoria Derbyshire, and Wei Wu.[2]

Early life and education[edit]

Wood received dual undergraduate degrees in biology and chemical engineering from the California Institute of Technology in 1990. He worked as an undergraduate in the lab led by Frances H. Arnold. He earned his Ph.D. in chemical engineering from Rensselaer Polytechnic Institute in 2000. His doctoral co-advisors were Georges Belfort at Rensselaer Polytechnic Institute, and Marlene Belfort at Wadsworth Center.[3]

Career[edit]

After obtaining his undergraduate degrees, Wood started working on high-viscosity mucopolysaccharide fermentation development at CP Kelco in San Diego, California. Soon after, he joined Amgen in 1991, the same year that Amgen received FDA approval for Neupogen. This recombinant protein cytokine drug induces white blood cell production to fight infections in immunocompromised cancer patients after undergoing chemotherapy or radiation treatment.[4] Wood worked on the GMP fermentation team to manufacture Neupogen (rhG-CSF) between 1991-1993. Neupogen became one of the most successful biotech drugs at that time and second blockbuster for Amgen after Epogen.[citation needed]

In 1993, Wood joined the group of Georges Belfort[5] as a Ph.D. student at Rensselaer Polytechnic Institute, where he worked primarily on mini-intein development for protein purification. During this time, he engineered the ∆I-CM intein, which was derived from Mycobacterium tuberculosis recA intein.[1][6][7][8] After earning his Ph.D. he joined a team at Bristol Myers Squibb in Hopewell, New Jersey. This time he focused on the recovery and in vitro processing of transgenic monoclonal antibody therapeutics.

In 2001, Wood started his academic career at Princeton University as an Assistant Professor of Chemical and Biological Engineering, where he continued his research focusing on self-removing tags, protein engineering, and applied biosensors.[9][10][11][12][13] In 2009, he joined the Chemical and Biomolecular Engineering Department at Ohio State University as an Associate Professor and soon after was appointed as a Full Professor.[citation needed][3] While at the Ohio State, he continued to explore additional applications, including the development of multitarget sRNAs that can be used for metabolic engineering, and the modification of human butyrylcholinesterase for the degradation of the chemical warfare nerve agents in collaboration with the Battelle Memorial Institute.[14][15]

Wood has joint appointments with Department of Chemistry and Biochemistry and the Molecular Biophysics Training Program at the Ohio State University. He is one of a small group of researchers worldwide focusing on intein implementation in various applications, along with Belfort and Tom Muir.[16][17]

Wood's research focuses on developing new technologies by recombining protein domains, particularly in biopharmaceutical development and manufacturing. He has continued refining these methods for biopharmaceutical development and manufacturing, and his work has drawn funding from the DARPA BioMOD project, NIH, NSF and US Army Research Office, projects as well as several industry sponsors.[18][19][20][21][22][23] Wood was also involved in the development of protein switches for biotechnology funded by the NSF Career Award.[24] Wood is an author of over 60 publications, six issued patents, two additional patent applications pending and an additional dozen book chapters or edited volumes. His publications have been cited nearly 4000 times.[25]

Wood is a member of the American Chemical Society, BIOT division, American Institute of Chemical Engineers, and International Society of Pharmaceutical Engineers.[citation needed][3]

Awards and honors[edit]

  • 2003: NSF Career Award[24]
  • 2016: Lumley Engineering Research Award, Ohio State College of Engineering[26]

Business[edit]

Protein Capture Science[27] is a biotech start-up company commercializing a self-removing affinity tag technology for research and large-scale purifications of proteins.[28][29] Wood and Izabela Gierach are co-founders of the company, based in Columbus, Ohio. The first line of the iCapTag products for protein purification was showcased in the Industry Innovators 2021-2022 Issue of the journal BioProcess International.[30] Protein Capture Science was recently awarded several grants and funding, including highly competitive The Ohio Third Frontier Technology Validation and Start-Up Fund and the Concept Fund for extraordinary technologies by the Department of Development, State of Ohio.[31]

References[edit]

  1. ^ a b Wood, David W.; Wu, Wei; Belfort, Georges; Derbyshire, Victoria; Belfort, Marlene (1999). "A genetic system yields self-cleaving inteins for bioseparations". Nature Biotechnology. 17 (9): 889–892. doi:10.1038/12879. PMID 10471931. S2CID 13247413.
  2. ^ US 6933362, Belfort, Marlene; Belfort, Georges & Derbyshire, Vicky et al., "Genetic system and self-cleaving inteins derived therefrom, bioseparations and protein purification employing same, and methods for determining critical, generalizable amino acid residues for varying intein activity", published 2005-08-23, assigned to Rensselaer Polytechnic Institute 
  3. ^ a b c "William G. Lowrie Department of Chemical and Biomolecular Engineering, David Wood biography, the Ohio State University". 17 July 2020.
  4. ^ "Amgen History". Amgen, Inc. Retrieved 2022-07-27.
  5. ^ "Georges Belfort | The Howard P. Isermann Department of Chemical and Biological Engineering". cbe.rpi.edu. Retrieved 2022-07-27.
  6. ^ Derbyshire, Victoria; Wood, David W.; Wu, Wei; Dansereau, John T.; Dalgaard, Jacob Z.; Belfort, Marlene (1997-10-14). "Genetic definition of a protein-splicing domain: Functional mini-inteins support structure predictions and a model for intein evolution". Proceedings of the National Academy of Sciences. 94 (21): 11466–11471. Bibcode:1997PNAS...9411466D. doi:10.1073/pnas.94.21.11466. PMC 23508. PMID 9326633.
  7. ^ Wood, D.W.; Derbyshire, V.; Wu, W.; Chartrain, M.; Belfort, M.; Belfort, G. (December 2000). "Optimized Single-Step Affinity Purification with a Self-Cleaving Intein Applied to Human Acidic Fibroblast Growth Factor". Biotechnology Progress. 16 (6): 1055–1063. doi:10.1021/bp0000858. PMID 11101334. S2CID 12491649.
  8. ^ Wood, David W. (2000). Generation and Application of a Self-cleaving Protein Linker for Use in Single-step Affinity Fusion Based Protein Purification (Thesis). OCLC 1150068137.[page needed]
  9. ^ Wu, Wan-Yi; Mee, Courtney; Califano, Filomena; Banki, Reza; Wood, David W. (2006). "Recombinant protein purification by self-cleaving aggregation tag". Nature Protocols. 1 (5): 2257–2262. doi:10.1038/nprot.2006.314. PMID 17406465. S2CID 24647545.
  10. ^ Skretas, G. (2005-02-01). "Regulation of protein activity with small-molecule-controlled inteins". Protein Science. 14 (2): 523–532. doi:10.1110/ps.04996905. PMC 2386410. PMID 15632292.
  11. ^ Skretas, Georgios; Meligova, Aggeliki K.; Villalonga-Barber, Carolina; Mitsiou, Dimitra J.; Alexis, Michael N.; Micha-Screttas, Maria; Steele, Barry R.; Screttas, Constantinos G.; Wood, David W. (July 2007). "Engineered Chimeric Enzymes as Tools for Drug Discovery: Generating Reliable Bacterial Screens for the Detection, Discovery, and Assessment of Estrogen Receptor Modulators". Journal of the American Chemical Society. 129 (27): 8443–8457. doi:10.1021/ja067754j. PMID 17569534.
  12. ^ Hartman, Izabela; Gillies, Alison R.; Arora, Sonia; Andaya, Christina; Royapet, Nitya; Welsh, William J.; Wood, David W.; Zauhar, Randy J. (October 2009). "Application of Screening Methods, Shape Signatures and Engineered Biosensors in Early Drug Discovery Process". Pharmaceutical Research. 26 (10): 2247–2258. doi:10.1007/s11095-009-9941-z. PMID 19626425. S2CID 3190373.
  13. ^ Li, Jingjing; Gierach, Izabela; Gillies, Alison R.; Warden, Charles D.; Wood, David W. (2011-11-15). "Engineering and optimization of an allosteric biosensor protein for peroxisome proliferator-activated receptor γ ligands". Biosensors and Bioelectronics. 29 (1): 132–139. doi:10.1016/j.bios.2011.08.006. PMC 3215401. PMID 21893405.
  14. ^ Lahiry, Ashwin; Stimple, Samuel D.; Wood, David W.; Lease, Richard A. (21 April 2017). "Retargeting a Dual-Acting sRNA for Multiple mRNA Transcript Regulation". ACS Synthetic Biology. 6 (4): 648–658. doi:10.1021/acssynbio.6b00261. PMID 28067500.
  15. ^ McGarry, Kevin G; Lalisse, Remy F; Moyer, Robert A; Johnson, Kristyn M; Tallan, Alexi M; Winters, Tyson P; Taris, Joeseph E; McElroy, Craig A; Lemmon, Erin E; Shafaat, Hannah S; Fan, Yamin; Deal, Aniliese; Marguet, Sean C; Harvilchuck, Jill A; Hadad, Christopher M; Wood, David W (March 2020). "A Novel, Modified Human Butyrylcholinesterase Catalytically Degrades the Chemical Warfare Nerve Agent, Sarin". Toxicological Sciences. 174 (1): 133–146. doi:10.1093/toxsci/kfz251. PMID 31879758.
  16. ^ Prabhala, Sai Vivek; Gierach, Izabela; Wood, David W. (2022). "The Evolution of Intein-Based Affinity Methods as Reflected in 30 years of Patent History". Frontiers in Molecular Biosciences. 9: 857566. doi:10.3389/fmolb.2022.857566. PMC 9033041. PMID 35463948.
  17. ^ Wood, David W.; Harcum, Sarah W.; Belfort, Georges (2005). "Industrial Applications of Intein Technology". Homing Endonucleases and Inteins. Nucleic Acids and Molecular Biology. Vol. 16. pp. 345–364. doi:10.1007/3-540-29474-0_20. ISBN 3-540-25106-5.
  18. ^ "NSF Award Search: Award # 1264322 - Highly Controllable Self-cleaving Tags for Biopharmaceutical Research and Manufacturing Purification Platforms". www.nsf.gov. Retrieved 2022-07-27.
  19. ^ "Just-in-time therapeutics manufacturing project receives additional DARPA funding". COLLEGE OF ENGINEERING. 2015-07-28. Retrieved 2022-07-27.
  20. ^ "Graduate students in the Frey Laboratory at UMBC". userpages.umbc.edu. Retrieved 2022-07-27.
  21. ^ "Ohio State engineering faculty attract $3.3 million in NSF funding in June". COLLEGE OF ENGINEERING. 2013-07-03. Retrieved 2022-07-27.
  22. ^ "Advancing the science and engineering of artificial blood". Chemical and Biomolecular Engineering. 2021-02-22. Retrieved 2022-07-27.
  23. ^ Banki, Mahmoud Reza; Feng, Liang; Wood, David W. (2005). "Simple bioseparations using self-cleaving elastin-like polypeptide tags". Nature Methods. 2 (9): 659–662. doi:10.1038/nmeth787. PMID 16074986. S2CID 15569524.
  24. ^ a b "NSF Award Search: Award # 0348220 - CAREER: Protein Switches for Molecular Biotechnology". www.nsf.gov. Retrieved 2022-07-27.
  25. ^ "David W. Wood". scholar.google.com. Retrieved 2022-07-27.
  26. ^ "David Wood's research recognized with Lumley Award". Chemical and Biomolecular Engineering. 2016-04-18. Retrieved 2022-07-27.
  27. ^ "Protein Capture Science". www.proteincapturescience.com. Retrieved 2022-07-27.
  28. ^ "Protein Capture Science Seeks to Accelerate Biomedical Research". Rev1 Ventures. 2021-05-20. Retrieved 2022-07-27.
  29. ^ "Protein Capture Science Accelerates Medical Therapies". Rev1 Ventures. 2022-04-15. Retrieved 2022-07-27.
  30. ^ "iCapTag Tagless Protein Purification Platform". BioProcess International. 2021-08-25. Retrieved 2022-07-27.
  31. ^ Ohio Advancing Cutting-Edge Technology, Third Frontier Accelerating New Ideas and Solutions.https://development.ohio.gov/static/business/thirdfrontier/2020%2006%2024%20Ohio%20Advancing%20Cutting-Edge%20Technology.pdf. Retrieved 2022-07-27

External links[edit]

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