Protein-coding gene in the species Homo sapiens
Nucleolysin TIAR is a protein that in humans is encoded by the TIAL1 gene .[5] [6]
The protein encoded by this gene is a member of a family of RNA -binding proteins, has three RNA recognition motifs (RRMs), and binds adenine and uridine -rich elements in mRNA and pre-mRNAs of a wide range of genes. It regulates various activities including translational control, splicing and apoptosis . Alternate transcriptional splice variants, encoding different isoforms , have been characterized. The different isoforms have been shown to function differently with respect to post-transcriptional silencing.[6]
References [ edit ] Further reading [ edit ] Taupin JL, Tian Q, Kedersha N, et al. (1995). "The RNA-binding protein TIAR is translocated from the nucleus to the cytoplasm during Fas-mediated apoptotic cell death" . Proc. Natl. Acad. Sci. U.S.A . 92 (5): 1629–33. Bibcode :1995PNAS...92.1629T . doi :10.1073/pnas.92.5.1629 PMC 42573 PMID 7533298 . Dember LM, Kim ND, Liu KQ, Anderson P (1996). "Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities" . J. Biol. Chem . 271 (5): 2783–8. doi :10.1074/jbc.271.5.2783 PMID 8576255 . Doi T, Minami T, Itoh M, et al. (1997). "An alternative form of nucleolysin binds to a T-cluster DNA in the silencer element of platelet factor 4 gene". Biochem. Biophys. Res. Commun . 235 (3): 625–30. doi :10.1006/bbrc.1997.6817 . PMID 9207209 . Jin K, Li W, Nagayama T, et al. (2000). "Expression of the RNA-binding protein TIAR is increased in neurons after ischemic cerebral injury". J. Neurosci. Res . 59 (6): 767–74. doi :10.1002/(SICI)1097-4547(20000315)59:6<767::AID-JNR9>3.0.CO;2-K . PMID 10700014 . S2CID 33671199 . Le Guiner C, Lejeune F, Galiana D, et al. (2001). "TIA-1 and TIAR activate splicing of alternative exons with weak 5' splice sites followed by a U-rich stretch on their own pre-mRNAs" . J. Biol. Chem . 276 (44): 40638–46. doi :10.1074/jbc.M105642200 PMID 11514562 . Iseni F, Garcin D, Nishio M, et al. (2002). "Sendai virus trailer RNA binds TIAR, a cellular protein involved in virus-induced apoptosis" . EMBO J . 21 (19): 5141–50. doi :10.1093/emboj/cdf513 . PMC 129035 PMID 12356730 . Li W, Li Y, Kedersha N, et al. (2002). "Cell proteins TIA-1 and TIAR interact with the 3' stem-loop of the West Nile virus complementary minus-strand RNA and facilitate virus replication" . J. Virol . 76 (23): 11989–2000. doi :10.1128/JVI.76.23.11989-12000.2002 . PMC 136884 PMID 12414941 . Yu Q, Cok SJ, Zeng C, Morrison AR (2003). "Translational repression of human matrix metalloproteinases-13 by an alternatively spliced form of T-cell-restricted intracellular antigen-related protein (TIAR)" . J. Biol. Chem . 278 (3): 1579–84. doi :10.1074/jbc.M203526200 PMID 12426321 . Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903. Bibcode :2002PNAS...9916899M . doi :10.1073/pnas.242603899 PMC 139241 PMID 12477932 . Le Guiner C, Gesnel MC, Breathnach R (2003). "TIA-1 or TIAR is required for DT40 cell viability" . J. Biol. Chem . 278 (12): 10465–76. doi :10.1074/jbc.M212378200 PMID 12533540 . Cok SJ, Acton SJ, Morrison AR (2003). "The proximal region of the 3'-untranslated region of cyclooxygenase-2 is recognized by a multimeric protein complex containing HuR, TIA-1, TIAR, and the heterogeneous nuclear ribonucleoprotein U." J. Biol. Chem . 278 (38): 36157–62. doi :10.1074/jbc.M302547200 PMID 12855701 . Zhu H, Hasman RA, Young KM, et al. (2003). "U1 snRNP-dependent function of TIAR in the regulation of alternative RNA processing of the human calcitonin/CGRP pre-mRNA" . Mol. Cell. Biol . 23 (17): 5959–71. doi :10.1128/MCB.23.17.5959-5971.2003 . PMC 180986 PMID 12917321 . Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs" . Nat. Genet . 36 (1): 40–5. doi :10.1038/ng1285 PMID 14702039 . Shukla S, Dirksen WP, Joyce KM, et al. (2004). "TIA proteins are necessary but not sufficient for the tissue-specific splicing of the myosin phosphatase targeting subunit 1" . J. Biol. Chem . 279 (14): 13668–76. doi :10.1074/jbc.M314138200 PMID 14736875 . Esclatine A, Taddeo B, Roizman B (2004). "Herpes simplex virus 1 induces cytoplasmic accumulation of TIA-1/TIAR and both synthesis and cytoplasmic accumulation of tristetraprolin, two cellular proteins that bind and destabilize AU-rich RNAs" . J. Virol . 78 (16): 8582–92. doi :10.1128/JVI.78.16.8582-8592.2004 . PMC 479066 PMID 15280467 . Hua Y, Zhou J (2004). "Survival motor neuron protein facilitates assembly of stress granules" . FEBS Lett . 572 (1–3): 69–74. doi :10.1016/j.febslet.2004.07.010 PMID 15304326 . S2CID 27599172 . Suswam EA, Nabors LB, Huang Y, et al. (2005). "IL-1beta induces stabilization of IL-8 mRNA in malignant breast cancer cells via the 3' untranslated region: Involvement of divergent RNA-binding factors HuR, KSRP and TIAR" . Int. J. Cancer . 113 (6): 911–9. doi :10.1002/ijc.20675 PMID 15514971 . S2CID 33272692 . Rush J, Moritz A, Lee KA, et al. (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol . 23 (1): 94–101. doi :10.1038/nbt1046 . PMID 15592455 . S2CID 7200157 . Suswam EA, Li YY, Mahtani H, King PH (2005). "Novel DNA-binding properties of the RNA-binding protein TIAR" . Nucleic Acids Res . 33 (14): 4507–18. doi :10.1093/nar/gki763 . PMC 1184220 PMID 16091628 . External links [ edit ] Overview of all the structural information available in the PDB for UniProt : Q01085 PDBe-KB .
PDB gallery
1x4g : Solution structure of RRM domain in Nucleolysin TIAR
2cqi : Solution structure of the RNA binding domain of Nucleolysin TIAR
2dgo : Solution structure of the RNA binding domain in cytotoxic granule-associated RNA binding protein 1
2dh7 : Solution structure of the second RNA binding domain in Nucleolysin TIAR
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