Corticosteroid 11-beta-dehydrogenase isozyme 2

HSD11B2
Identifiers
Aliases	HSD11B2, AME, AME1, HSD11K, HSD2, SDR9C3, hydroxysteroid (11-beta) dehydrogenase 2, hydroxysteroid 11-beta dehydrogenase 2
External IDs	OMIM: 614232; MGI: 104720; HomoloGene: 20088; GeneCards: HSD11B2; OMA:HSD11B2 - orthologs
Gene location (Human)
Chr.	Chromosome 16 (human)
End	67,437,553 bp
Gene location (Mouse)
Chr.	Chromosome 8 (mouse)
End	106,250,620 bp
RNA expression pattern
	Top expressed in
	mucosa of transverse colon; ; renal medulla; ; rectum; ; human kidney; ; mucosa of ileum; ; parotid gland; ; skin of leg; ; mucosa of sigmoid colon; ; skin of abdomen; ; minor salivary glands;
	Top expressed in
	left colon; ; right kidney; ; uterus; ; yolk sac; ; human kidney; ; otic vesicle; ; cervix; ; saccule; ; Gonadal ridge; ; abdominal wall;
	More reference expression data
	More reference expression data
Gene ontology
Molecular function	NAD binding; steroid binding; oxidoreductase activity; 11-beta-hydroxysteroid dehydrogenase [NAD(P) activity];
Cellular component	cytoplasm; endoplasmic reticulum membrane; intracellular membrane-bounded organelle; endoplasmic reticulum;
Biological process	response to hypoxia; glucocorticoid metabolic process; female pregnancy; response to steroid hormone; response to glucocorticoid; response to insulin; glucocorticoid biosynthetic process; regulation of blood volume by renal aldosterone; response to food;
	Sources:Amigo / QuickGO
Orthologs
	3291
	15484
	ENSG00000176387
	ENSMUSG00000031891
	P80365
	P51661
	NM_000196
	NM_008289
	NP_000187
	NP_032315
	Wikidata
View/Edit Human	View/Edit Mouse

Corticosteroid 11-β-dehydrogenase isozyme 2 also known as 11-β-hydroxysteroid dehydrogenase 2 is an enzyme that in humans is encoded by the HSD11B2 gene.^[5]^[6]^[7]

Function

Corticosteroid 11-β-dehydrogenase isozyme 2 is an NAD⁺-dependent enzyme expressed in aldosterone-selective epithelial tissues such as the kidney, colon, salivary and sweat glands. HSD211B2 expression is also found in the brainstem in a small, aldosterone-sensitive subset of neurons located in the nucleus of the solitary tract referred to as HSD2 neurons.^[8]

In these tissues, HSD11B2 oxidizes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. This protective mechanism is necessary because cortisol circulates at 100- to 1000-fold higher concentrations than aldosterone, and binds with equal affinity to the mineralocorticoid receptor, thereby out-competing aldosterone in cells that do not produce HSD11B2.

This glucocorticoid-inactivating enzyme is also expressed in tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, as well as parts of the developing brain, including the rhombencephalic progenitor cells that proliferate into cerebellar granule cells. In these tissues, HSD11B2 protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development.

Clinical significance

Inhibition of this enzyme, for example by the compound glycyrrhetinic acid enzymatically converted from glycyrrhizic acid, found in natural liquorice, results in a condition known as pseudohyperaldosteronism. A genetically inherited deficiency of HSD11B2 is the underlying cause of the syndrome of apparent mineralocorticoid excess.

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000176387 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000031891 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Albiston AL, Obeyesekere VR, Smith RE, Krozowski ZS (November 1994). "Cloning and tissue distribution of the human 11 beta-hydroxysteroid dehydrogenase type 2 enzyme". Mol. Cell. Endocrinol. 105 (2): R11–7. doi:10.1016/0303-7207(94)90176-7. PMID 7859916. S2CID 8240801.
^ Brown RW, Chapman KE, Kotelevtsev Y, Yau JL, Lindsay RS, Brett L, Leckie C, Murad P, Lyons V, Mullins JJ, Edwards CR, Seckl JR (February 1996). "Cloning and production of antisera to human placental 11 beta-hydroxysteroid dehydrogenase type 2". Biochem. J. 313 (Pt 3): 1007–17. doi:10.1042/bj3131007. PMC 1216963. PMID 8611140.
^ "Entrez Gene: HSD11B2 hydroxysteroid (11-beta) dehydrogenase 2".
^ Geerling, Joel C.; Arthur D. Loewy (September 2009). "Aldosterone in the brain". American Journal of Physiology. Renal Physiology. 297 (3): F559–76. doi:10.1152/ajprenal.90399.2008. PMC 2739715. PMID 19261742.

White PC, Mune T, Agarwal AK (1997). "11 beta-Hydroxysteroid dehydrogenase and the syndrome of apparent mineralocorticoid excess". Endocr. Rev. 18 (1): 135–56. doi:10.1210/edrv.18.1.0288. PMID 9034789.
Wilson RC, Dave-Sharma S, Wei JQ, et al. (1998). "A genetic defect resulting in mild low-renin hypertension". Proc. Natl. Acad. Sci. U.S.A. 95 (17): 10200–5. Bibcode:1998PNAS...9510200W. doi:10.1073/pnas.95.17.10200. PMC 21485. PMID 9707624.
Quinkler M, Stewart PM (2003). "Hypertension and the cortisol-cortisone shuttle". J. Clin. Endocrinol. Metab. 88 (6): 2384–92. doi:10.1210/jc.2003-030138. PMID 12788832.
Tomlinson JW, Walker EA, Bujalska IJ, et al. (2005). "11beta-hydroxysteroid dehydrogenase type 1: a tissue-specific regulator of glucocorticoid response". Endocr. Rev. 25 (5): 831–66. doi:10.1210/er.2003-0031. PMID 15466942.
Persu A (2005). "11beta-Hydroxysteroid deshydrogenase: a multi-faceted enzyme". J. Hypertens. 23 (1): 29–31. doi:10.1097/00004872-200501000-00007. PMID 15643119.
Funder JW, Pearce PT, Smith R, Smith AI (1988). "Mineralocorticoid action: target tissue specificity is enzyme, not receptor, mediated". Science. 242 (4878): 583–5. Bibcode:1988Sci...242..583F. doi:10.1126/science.2845584. PMID 2845584.
Stewart PM, Wallace AM, Valentino R, et al. (1987). "Mineralocorticoid activity of liquorice: 11-beta-hydroxysteroid dehydrogenase deficiency comes of age". Lancet. 2 (8563): 821–4. doi:10.1016/S0140-6736(87)91014-2. PMID 2889032. S2CID 42872913.
Wilson RC, Harbison MD, Krozowski ZS, et al. (1995). "Several homozygous mutations in the gene for 11 beta-hydroxysteroid dehydrogenase type 2 in patients with apparent mineralocorticoid excess". J. Clin. Endocrinol. Metab. 80 (11): 3145–50. doi:10.1210/jcem.80.11.7593417. PMID 7593417.
Wilson RC, Krozowski ZS, Li K, et al. (1995). "A mutation in the HSD11B2 gene in a family with apparent mineralocorticoid excess". J. Clin. Endocrinol. Metab. 80 (7): 2263–6. doi:10.1210/jcem.80.7.7608290. PMID 7608290.
Krozowski Z, Baker E, Obeyesekere V, Callen DF (1995). "Localization of the gene for human 11 beta-hydroxysteroid dehydrogenase type 2 (HSD11B2) to chromosome band 16q22". Cytogenet. Cell Genet. 71 (2): 124–5. doi:10.1159/000134089. PMID 7656579.
Mune T, Rogerson FM, Nikkilä H, et al. (1995). "Human hypertension caused by mutations in the kidney isozyme of 11 beta-hydroxysteroid dehydrogenase". Nat. Genet. 10 (4): 394–9. doi:10.1038/ng0895-394. PMID 7670488. S2CID 30848352.
Agarwal AK, Rogerson FM, Mune T, White PC (1996). "Gene structure and chromosomal localization of the human HSD11K gene encoding the kidney (type 2) isozyme of 11 beta-hydroxysteroid dehydrogenase". Genomics. 29 (1): 195–9. doi:10.1006/geno.1995.1231. PMID 8530071.
Krozowski Z, Albiston AL, Obeyesekere VR, et al. (1996). "The human 11 beta-hydroxysteroid dehydrogenase type II enzyme: comparisons with other species and localization to the distal nephron". J. Steroid Biochem. Mol. Biol. 55 (5–6): 457–64. doi:10.1016/0960-0760(95)00194-8. PMID 8547170. S2CID 12550923.
Brown RW, Chapman KE, Murad P, et al. (1996). "Purification of 11 beta-hydroxysteroid dehydrogenase type 2 from human placenta utilizing a novel affinity labelling technique". Biochem. J. 313 (Pt 3): 997–1005. doi:10.1042/bj3130997. PMC 1217009. PMID 8611186.
Kitanaka S, Katsumata N, Tanae A, et al. (1998). "A new compound heterozygous mutation in the 11 beta-hydroxysteroid dehydrogenase type 2 gene in a case of apparent mineralocorticoid excess". J. Clin. Endocrinol. Metab. 82 (12): 4054–8. doi:10.1210/jcem.82.12.4455. PMID 9398712.
Dave-Sharma S, Wilson RC, Harbison MD, et al. (1998). "Examination of genotype and phenotype relationships in 14 patients with apparent mineralocorticoid excess". J. Clin. Endocrinol. Metab. 83 (7): 2244–54. doi:10.1210/jcem.83.7.4986. PMID 9661590.
Li A, Tedde R, Krozowski ZS, et al. (1998). "Molecular basis for hypertension in the "type II variant" of apparent mineralocorticoid excess". Am. J. Hum. Genet. 63 (2): 370–9. doi:10.1086/301955. PMC 1377297. PMID 9683587.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000176387 – Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000031891 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid7859916-5] Albiston AL, Obeyesekere VR, Smith RE, Krozowski ZS (November 1994). "Cloning and tissue distribution of the human 11 beta-hydroxysteroid dehydrogenase type 2 enzyme". Mol. Cell. Endocrinol. 105 (2): R11–7. doi:10.1016/0303-7207(94)90176-7. PMID 7859916. S2CID 8240801.

[pmid8611140-6] Brown RW, Chapman KE, Kotelevtsev Y, Yau JL, Lindsay RS, Brett L, Leckie C, Murad P, Lyons V, Mullins JJ, Edwards CR, Seckl JR (February 1996). "Cloning and production of antisera to human placental 11 beta-hydroxysteroid dehydrogenase type 2". Biochem. J. 313 (Pt 3): 1007–17. doi:10.1042/bj3131007. PMC 1216963. PMID 8611140.

[entrez-7] "Entrez Gene: HSD11B2 hydroxysteroid (11-beta) dehydrogenase 2".

[8] Geerling, Joel C.; Arthur D. Loewy (September 2009). "Aldosterone in the brain". American Journal of Physiology. Renal Physiology. 297 (3): F559–76. doi:10.1152/ajprenal.90399.2008. PMC 2739715. PMID 19261742.

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v t e Oxidoreductases: alcohol oxidoreductases (EC 1.1)
1.1.1: NAD/NADP acceptor	3-hydroxyacyl-CoA dehydrogenase 3-hydroxybutyryl-CoA dehydrogenase Alcohol dehydrogenase Aldo-keto reductase 1A1 1B1 1B10 1C1 1C3 1C4 7A2 Aldose reductase Beta-Ketoacyl ACP reductase Carbohydrate dehydrogenases Carnitine dehydrogenase D-malate dehydrogenase (decarboxylating) DXP reductoisomerase Glucose-6-phosphate dehydrogenase Glycerol-3-phosphate dehydrogenase HMG-CoA reductase IMP dehydrogenase Isocitrate dehydrogenase Lactate dehydrogenase L-threonine dehydrogenase L-xylulose reductase Malate dehydrogenase Malate dehydrogenase (decarboxylating) Malate dehydrogenase (NADP+) Malate dehydrogenase (oxaloacetate-decarboxylating) Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) Phosphogluconate dehydrogenase Sorbitol dehydrogenase Hydroxysteroid dehydrogenase: 3β 3β-HSD NSDHL 11β 11β-HSD1 11β-HSD2 17β
1.1.2: cytochrome acceptor	D-lactate dehydrogenase (cytochrome) D-lactate dehydrogenase (cytochrome c-553) Mannitol dehydrogenase (cytochrome)
1.1.3: oxygen acceptor	Glucose oxidase L-gulonolactone oxidase Xanthine oxidase Alcohol oxidase
1.1.4: disulfide as acceptor	Vitamin K epoxide reductase Vitamin-K-epoxide reductase (warfarin-insensitive)
1.1.5: quinone/similar acceptor	Malate dehydrogenase (quinone) Quinoprotein glucose dehydrogenase
1.1.99: other acceptors	Choline dehydrogenase L2HGDH

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)

Corticosteroid 11-beta-dehydrogenase isozyme 2

Function

Clinical significance

References

Further reading

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