Protein-coding gene in the species Homo sapiens
Ribonuclease H1 also known as RNase H1 is an enzyme that in humans is encoded by the RNASEH1 gene .[5] [6] [7] endonuclease and catalyzes the cleavage of RNA via a hydrolytic mechanism.
References [ edit ] ^ a b c GRCh38: Ensembl release 89: ENSG00000171865 – Ensembl , May 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000020630 – Ensembl , May 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Entrez Gene: ribonuclease H1" .^ ten Asbroek AL, van Groenigen M, Jakobs ME, Koevoets C, Janssen B, Baas F (June 2002). "Ribonuclease H1 maps to chromosome 2 and has at least three pseudogene loci in the human genome". Genomics . 79 (6): 818–23. doi :10.1006/geno.2002.6776 . PMID 12036296 . ^ Nowotny M, Gaidamakov SA, Ghirlando R, Cerritelli SM, Crouch RJ, Yang W (October 2007). "Structure of human RNase H1 complexed with an RNA/DNA hybrid: insight into HIV reverse transcription" . Mol. Cell . 28 (2): 264–76. doi :10.1016/j.molcel.2007.08.015 PMID 17964265 . Further reading [ edit ] Nowotny M, Cerritelli SM, Ghirlando R, et al. (2008). "Specific recognition of RNA/DNA hybrid and enhancement of human RNase H1 activity by HBD" . EMBO J . 27 (7): 1172–81. doi :10.1038/emboj.2008.44 . PMC 2323259 PMID 18337749 . Lima WF, Wu H, Nichols JG, et al. (2003). "Human RNase H1 uses one tryptophan and two lysines to position the enzyme at the 3'-DNA/5'-RNA terminus of the heteroduplex substrate" . J. Biol. Chem . 278 (50): 49860–7. doi :10.1074/jbc.M306543200 PMID 14506260 . Lima WF, Rose JB, Nichols JG, et al. (2007). "The positional influence of the helical geometry of the heteroduplex substrate on human RNase H1 catalysis". Mol. Pharmacol . 71 (1): 73–82. doi :10.1124/mol.106.025429 . PMID 17028157 . S2CID 27765676 . Lima WF, Nichols JG, Wu H, et al. (2004). "Structural requirements at the catalytic site of the heteroduplex substrate for human RNase H1 catalysis" . J. Biol. Chem . 279 (35): 36317–26. doi :10.1074/jbc.M405035200 PMID 15205459 . Otsuki T, Ota T, Nishikawa T, et al. (2005). "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries" . DNA Res . 12 (2): 117–26. doi :10.1093/dnares/12.2.117 PMID 16303743 . Wu H, Lima WF, Crooke ST (1999). "Properties of cloned and expressed human RNase H1" . J. Biol. Chem . 274 (40): 28270–8. doi :10.1074/jbc.274.40.28270 PMID 10497183 . Frank P, Braunshofer-Reiter C, Pöltl A, Holzmann K (1998). "Cloning, subcellular localization and functional expression of human RNase HII". Biol. Chem . 379 (12): 1407–12. doi :10.1515/bchm.1998.379.12.1407 . PMID 9894807 . S2CID 19692186 . Wu H, Lima WF, Zhang H, et al. (2004). "Determination of the role of the human RNase H1 in the pharmacology of DNA-like antisense drugs" . J. Biol. Chem . 279 (17): 17181–9. doi :10.1074/jbc.M311683200 PMID 14960586 . Hillier LW, Graves TA, Fulton RS, et al. (2005). "Generation and annotation of the DNA sequences of human chromosomes 2 and 4" . Nature . 434 (7034): 724–31. Bibcode :2005Natur.434..724H . doi :10.1038/nature03466 PMID 15815621 . Lima WF, Wu H, Nichols JG, et al. (2003). "Human RNase H1 activity is regulated by a unique redox switch formed between adjacent cysteines" . J. Biol. Chem . 278 (17): 14906–12. doi :10.1074/jbc.M211279200 PMID 12473655 . ten Asbroek AL, van Groenigen M, Nooij M, Baas F (2002). "The involvement of human ribonucleases H1 and H2 in the variation of response of cells to antisense phosphorothioate oligonucleotides" . Eur. J. Biochem . 269 (2): 583–92. doi :10.1046/j.0014-2956.2001.02686.x PMID 11856317 . Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)" . Genome Res . 14 (10B): 2121–7. doi :10.1101/gr.2596504 . PMC 528928 PMID 15489334 . Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs" . Nat. Genet . 36 (1): 40–5. doi :10.1038/ng1285 PMID 14702039 . Cerritelli SM, Crouch RJ (1998). "Cloning, expression, and mapping of ribonucleases H of human and mouse related to bacterial RNase HI" . Genomics . 53 (3): 300–7. doi :10.1006/geno.1998.5497 . PMID 9799596 . Strausberg RL, Feingold EA, Grouse LH, et al. (2002). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903. Bibcode :2002PNAS...9916899M . doi :10.1073/pnas.242603899 PMC 139241 PMID 12477932 . Cerritelli SM, Frolova EG, Feng C, et al. (2003). "Failure to produce mitochondrial DNA results in embryonic lethality in Rnaseh1 null mice" . Mol. Cell . 11 (3): 807–15. doi :10.1016/S1097-2765(03)00088-1 PMID 12667461 . Lima WF, Rose JB, Nichols JG, et al. (2007). "Human RNase H1 discriminates between subtle variations in the structure of the heteroduplex substrate". Mol. Pharmacol . 71 (1): 83–91. doi :10.1124/mol.106.025015 . PMID 17028158 . S2CID 24995048 . External links [ edit ] Overview of all the structural information available in the PDB for UniProt : O60930 PDBe-KB .
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