tRNA (guanine-N2-)-methyltransferase

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In enzymology, a tRNA (guanine-N2-)-methyltransferase (EC 2.1.1.32) is an enzyme that catalyzes the chemical reaction

S-adenosyl-L-methionine + tRNA ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + tRNA containing N₂-methylguanine

Thus, the two substrates of this enzyme are S-adenosyl methionine and tRNA, whereas its two products are S-adenosylhomocysteine and tRNA containing N2-Methylguanine.

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:tRNA (guanine-N2-)-methyltransferase. Other names in common use include transfer ribonucleate guanine 2-methyltransferase, transfer ribonucleate guanine N2-methyltransferase, transfer RNA guanine 2-methyltransferase, guanine-N2-methylase, and S-adenosyl-L-methionine:tRNA (guanine-2-N-)-methyltransferase.

Structural studies[edit]

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 2DUL, 2EJT, and 2EJU.

References[edit]

• Baguley BC, Staehelin M (1968). "The specificity of transfer ribonucleic acid methylases from rat liver". Biochemistry. 7 (1): 45–50. doi:10.1021/bi00841a007. PMID 4921283.
• Bjork GR, Svensson I (1969). "Studies on microbial RNA. Fractionation of tRNA methylases from Saccharomyces cerevisiae". Eur. J. Biochem. 9 (2): 207–15. doi:10.1111/j.1432-1033.1969.tb00596.x. PMID 4896260.
• Krauss J, Stahelin M (1974). "N2-Guanine specific transfer RNA methyltransferase I from rat liver and leukemic rat spleen*". Nucleic Acids Res. 1 (11): 1455–1478. doi:10.1093/nar/1.11.1455. PMC 343424. PMID 10793703.
• Krauss J, Stahelin M (1974). "N2-Guanine specific transfer RNA methyltransferase II from rat liver". Nucleic Acids Res. 1 (11): 1479–1496. doi:10.1093/nar/1.11.1479. PMC 343425. PMID 10793704.
• Agris PF, Spremulli LL, Brown GM (1974). "tRNA methylases from HeLa cells: purification and properties of an adenine-1-methylase and a guanine-N2-methylase". Arch. Biochem. Biophys. 162 (1): 38–47. doi:10.1016/0003-9861(74)90102-7. PMID 4208689.
• Glick JM, Ross S, Leboy PS (1975). "S-adenosylhomocysteine inhibition of three purified tRNA methyltransferases from rat liver". Nucleic Acids Res. 2 (10): 1639–51. doi:10.1093/nar/2.10.1639. PMC 343534. PMID 1208211.
• Izzo P, Gantt R (1977). "Partial purification and characterization of an N2-guanine RNA methyltransferase from chicken embryos". Biochemistry. 16 (16): 3576–81. doi:10.1021/bi00635a012. PMID 19056.
• Glick JM, Averyhart VM, Leboy PS (1978). "Purification and characterization of two tRNA-(guanine)-methyltransferases from rat liver". Biochim. Biophys. Acta. 518 (1): 158–71. doi:10.1016/0005-2787(78)90125-9. PMID 629973.